ESI offers an extensive variety of environmental risk assessment testing services designed to evaluate the impact of pollutants on various habitats.
ESI provides a full range of analytical chemistry services directed toward the analysis of soil, sediment, water and tissue matrices.
Frog Embryo Teratogenesis Assay Xenopus (FETAX) assays demonstrating potential adverse ecological impacts by monitoring development and growth of eggs and tadpoles.
Freshwater toxicity testing programs incorporating acute and chronic exposure assays with, amphipods, midge larvae, daphnids, minnows, and aquatic worms
Testing services specifically designed to address issues related to detecting and quantifying toxicity of soils, sediments, leachates, and hazardous wastes
Marine dredged sediment testing programs and analytical chemistry services specifically designed to address issues related to permitting and monitoring of dredging and disposal projects.
Extraction, recovery, and reliable detection of organic analytes from tissue samples to document body burdens.
WET (Whole Effluent Testing) WER (Water Effects Ratio) studies designed to support the toxicological testing and analytical chemical programs requirements specified in State and Federal wastewater discharge permits.
Anaylsis of Contaminants of Concern (COCs) in sediment pore waters and elutrate solutions.
EnviroSystems, Inc. provides an extensive variety of environmental testing services designed to evaluate the impact of pollutants on various habitats. These services include: acute and chronic toxicological evaluations of; soils, sediments, effluents and leachates. Studies are designed specifically to address toxicological and bioaccumulative potential, standard drill mud and dispersant toxicity tests; on-site WET (Whole Effluent Testing) requirements, WER (Water Effects Ratio) studies, or in-stream toxicity and bioconcentration tests; sediment bioaccumulation studies; and fish avoidance studies.
All environmental toxicity and bioconcentration tests are available with standard or specially selected marine and freshwater fish, invertebrates and plants. Tests can be conducted under static, renewal or flow-through exposure conditions with natural or artificial freshwater and saltwater, depending on the required protocol.
EnviroSystems, Inc. provides a full range of analytical chemistry services directed toward the analysis of soil, sediment, water and tissue matrices. ESI has tailored services specifically to address issues related to analysis of trace metals and organic compounds in sediments, soils, waters, tissues and plants for over 30 years.
EnviroSystems’ laboratory is equipped with automated instrumentation for the preparation and analysis of sediments, soils, waters and tissue samples. The laboratory functions in accordance with the standards of the National Environmental Laboratory Accreditation Program (NELAP) accreditation.
In dealing with environmental samples special attention is paid by EnviroSystems’ chemists to recognize that standard reporting limits may exceed risk-based limits for some compounds of concern. By using a project-specific approach, analysts can apply the most sensitive techniques to achieve the lowest reporting limits and best quantitation for each study.
The EnviroSystems analytical chemistry team’s mission is to support each client’s analytical service needs with responsive, accurate and defensible analytical data. Close interaction of our biologists and chemists with field project scientists and engineers, produces data that consistently meets or exceeds the data quality objectives of environmental investigation and compliance monitoring programs.
In addition to custom testing, ESI offers routine tests for:
EnviroSystems, Inc. provides a full range of environmental toxicological and analytical chemistry testing services specifically designed to address issues related to detecting and quantifying toxicity of leachates, surface waters and sediments. Amphibian evaluations are conducted using Frog Embryo Teratogenesis Assay Xenopus, or FETAX assays.
FETAX assays demonstrate potential adverse ecological impacts by monitoring development and growth of eggs and tadpoles. Newly fertilized frog eggs are exposed to a range of sample types including, leachates, surface waters and sediments. Embryonic development is monitored along with tadpole growth and development to assess teratogenic developmental effects and chronic, sublethal effects on growth. Incorporation of FETAX assays into overall testing schemes designed to evaluate potential toxicity of leachates, ground water, surface waters and sediments is an additional tool to assess potential impacts. The assay generates both acute and chronic exposure endpoints for establishing site prioritization, characterization or for documentation of cleanup success and monitoring.
Adult frogs with fertilized eggs, male frog is the smaller of the pair.
Frog embryos at the start of assay.
Tadpoles during the assay after 24 hours of exposure.
Tadpoles at the end of the assay after 96 hours of exposure.
EnviroSystems, Inc. provides a full range of environmental toxicological and analytical chemistry testing services specifically designed to address issues related to detecting and quantifying toxicity of sediments and hazardous wastes. ESI’s facility is designed so it can simultaneously evaluate over 100 samples with varying environmental conditions.
Freshwater toxicity testing programs incorporate acute and chronic exposure assays with, amphipods, midge larvae, daphnids, minnows and aquatic worms. Data generated from these programs are being used to document site prioritization and characterization, waste characterization, cleanup standards and site monitoring. Analytical chemistry provides for both sediment and tissue analysis. ESI’s scientists work with our clients to design program sampling and analysis plans to achieve data quality objectives. Toxicity and bioaccumulation testing programs are based on EPA and ASTM standard protocol.
Emergence traps used in midge, Chironomus dilutus, day 35 of life cycle assay.
Midge larvae recovered on day 28 of life cycle assay.
In addition to custom testing, ESI offers routine tests for:
Egg case produced on day 48 of life cycle assay.
Freshwater oligochaetes, Lumbriculus variegatus, recovered at end of 28-day bioaccumulation evaluation.
EnviroSystems, Inc. provides a full range of environmental toxicological and analytical chemistry testing services specifically designed to address issues related to detecting and quantifying toxicity of soils, sediments, leachates, and hazardous wastes. ESI’s analytical group also provides comprehensive chemical analysis of tissue samples, either from laboratory bioaccumulation evaluations or field collection programs.
Toxicity programs for evaluation of hazardous materials, including soils and sludges, based on the responses of vascular plants and soil invertebrates, generates information based on sensitive endpoints in relatively short periods. These data also have benefits over a purely chemical specific evaluation of wastes as the information can be used to draw direct conclusions as to potential impacts.
Rye grass, Lollium perenne, recovered on day 28 of chronic exposure assay.
Mature Eisenia fetida recovered at end of 28-day survival and growth assay.
Flowered plants at end of 42-day Life Cycle Assay using Brassica rapa.
EnviroSystems, Inc. provides a full range of toxicological testing programs and analytical chemistry services specifically designed to address issues related to permitting and monitoring of dredging and disposal projects. ESI has conducted Tier III sediment evaluations for private sector projects and Federal navigation maintenance projects at locations ranging from Maine to Mississippi to Puerto Rico.
Laboratory services are routinely available from ESI to cover all aspects for dredged material testing associated with Tier II, Bulk Sediment Analysis, through Tier III, acute sediment toxicity, water column analysis, Suspended Particulate Phase assays and elutriate analysis and Bioaccumulation Testing.
Envirosystems’ facility has been designed to accommodate the full range of testing services necessary to assess impacts of dredged sediments on both marine and freshwater environments. The facility is capable of conducting concurrent testing programs under multiple environments, allowing for the control of temperature, salinity and photoperiod.
While there is no State or Federal certification of laboratories conducting dredged sediment evaluations, ESI has an approved LQAP on file with the New England District Corps of Engineers covering all biological and analytical services necessary for conducting bulk sediment, biological, elutriate and bioaccumulation testing.
10 Day Solid Phase Assay.
Elutriate / Suspended Particulate Phase Preparation.
Sea Urchin Larvae – Suspended Particulate Phase Assay.
PCB congener analysis using high resolution GC-MS Specific Ion Monitoring.
EnviroSystems, Inc. provides a full range of analytical chemistry services directed toward the analysis of soil, sediment, water and tissue matrices. ESI has tailored services specifically to address issues related to uptake of trace metals and organic compounds by invertebrates, vertebrates and plants for over 30 years.
Analysis of tissue samples to document body burdens poses a range of challenges not normally found in routine environmental samples. Extraction, recovery, and reliable detection of organic analytes from biogenic material requires the best equipment in the hands of specialists. Standard analytical methods must be optimized for biological samples to maintain responsive reporting limits in small sample volumes. EnviroSystems’ analytical protocols address these issues.
Routine analyses available for evaluation of tissue samples include; trace metals, PAH compounds, PCB congeners, pesticides, explosive residue and semi-volatile organic compounds.
|Trace Metals||ICP-MS / ICP-OES||EPA 200.8 / 200.7|
|PAHs||GC-MS||EPA 8270 SIM|
|PCB Congeners||GC-MS||EPA 680 SIM|
|Explosive Residue||GC-MS||EPA 680 SIM|
EnviroSystems, Inc. provides an extensive range of toxicological testing services directly related to supporting biomonitoring requirements specified in State and Federal wastewater discharge permits issued in compliance with the Clean Water Act’s National Pollution Discharge Elimination Scheme (NPDES).
EnviroSystems has provided toxicological and analytical chemistry services to support Water Effects Ratio (WER) studies for more than 30 years. Studies have been completed for both individual clients and consortiums of dischargers working together to provide documentation for establishing either discharge specific or basin wide permit limits.
ESI’s facility has been specifically designed to support toxicological testing and analytical chemical programs necessary to meet all our client’s monitoring needs at a single location. For over 30 years ESI has provided NPDES biomonitoring support and services to clients throughout the continental United States as well as locations in Puerto Rico and America Samoa. ESI is NELAC accredited through the State of New Hampshire for a full ranges of toxicological and analytical parameters.
EnviroSystems, Inc. provides tailored services specifically to address issues related to levels of Contaminants of Concern (COCs) in the water column.
ESI’s services include the capability to provide a full range of analytical services for elutriate analysis for both freshwater and marine samples. U.S. Army Corps of Engineers’ Evaluation of Dredged Material Proposed for Discharge in Waters of the U.S., Inland Testing Manual, and Evaluation of Dredged Material Proposed for Disposal at Island, Nearshore, or Upland Confined Disposal Facilities guidance documents specify use of elutriate testing procedures for assessing levels of Contaminants of Concern (COCs) in water column samples.
Passive samplers are being used to provide data on levels of Contaminants of Concern (COCs) in pore waters during acute and chronic exposure assays. ESI provides a range of passive sampling devices for direct deployment in assay test chambers. ESI also provides a full range of supporting chemistry services associated with sampler analysis. Sampler design is developed in conjunction with project staff to ensure that project scope of work and quality assurance plan objectives are achieved.
Please call for information on current MDLs, project specific reporting limits or for a quote.
To assess pore water trace metal concentrations, ESI designs and constructs passive pore-water samplers, "Peepers" using current best practices and take into account test chamber sediment volume and available pore water content. Peepers can be constructed of either polyethylene or glass. A polyethersulfone membrane, 0.45 µm pore diameter, separates outside sediments from the deoxygenated deionized water in the chamber. Peeper components are assembled in deoxygenated deionized water in small batches. Use of deoxygenated deionized water mitigates formation of metallic oxides which can clog the membrane. To further mitigate impacts of oxygen in the peepers units are prepared just prior to use. Assembled units are subjected to quality assurance analysis for COCs prior to use. Individual peeper volumes generally range from 2.0 to 4 mL and multiple samplers may be deployed in a chamber to increase final sample volume. Direct analysis of the peeper contents, using ICP-MS methods, is available from our in-house chemistry group.
ESI routinely generates both standard and modified elutriate solutions for freshwater and marine waters and sediments. Standard elutriate solutions are prepared in glass or Teflon® lined stainless steel vessels incorporating Teflon® coated stainless steel stirring apparatus. Modified elutriate solution preparation utilizes the same containers with laboratory grade compressed air providing the mixing. Systems are available to provide elutriate volumes that range from 1 to 42 liters at a single time. The maximum volumes or standard and modified elutriate solution that can simultaneously be generated using glass/Teflon® systems is 40 liters while the all Teflon® coated stainless steel system is capable of generating 70 liters. The laboratory can prepare multiple batches each day.
After preparation, elutriate solutions are centrifuged to remove solids using high capacity/high volume refrigerated centrifuges. High volume units (4 to 6 Liters/run) are capable of providing a Relative Centrifical Force (RCF) of over 6,000 g while lower volume units (1.5 Liters/run) provide an RCF of over 15,000 g. Subsequent membrane filtration, for inorganic parameters, is also available.
Solid Phase Micro Extraction (SPME) fibers are used to monitor organic pore water constituents. ESI obtains SPME fibers from commercial sources with options for the fiber’s organic coating. ESI’s technical staff can provide technical assistance to optimize fiber length and coating plus number of fibers assigned to a test chamber. Fibers may be deployed directly in the sediment or in a protective mesh envelope during the assay. At the termination of an assay, lengths of the recovered fibers are documented. Fibers can then be transferred to appropriate chambers for extraction of COCs and subsequent analysis.